Current projects

Clarification of actin, NADPH oxidase and structural sterol roles in root hair tip growth using advanced microscopy and proteomics

GACR; PI J. Šamaj

Studies on developmental roles of iron superoxide dismutase 1 using interdisciplinary approach

GACR; PI T. Takáč

Plants as a tool for sustainable global development

Operational Programme Research, Development and Education;PI I. Frébort (CATRIN)scientific coordinator J. Šamaj

Role of MAPK signalling and metabolomic reprograming during Arabidopsis-Xanthomonas interaction

Research Group Linkage Programme, Humboldt Foundation; PI J. Šamaj

Plant proteomics: cytoskeleton, membranes and signalling

Former Projects

Genetic and cell biology approaches to study regulation of YODA (MAP3K4) signaling by HSP90 proteins in Arabidopsis

GACR; PI J. Šamaj; project with excelent evaluations

Revisiting microtubule organization during plant cell division, growth and morphogenesis by superresolution microscopy

GACR; PI G. Komis

Regulation of phospholipase D alpha 1 function by MPK3-dependent phosphorylation

GACR; PI J. Šamaj

Study of KATANIN1 phosphorylation and microtubules fission in Arabidopsis

GACR; PI J. Šamaj

The role of mitogen-activated protein kinases (MPKs) in barley responses to Puccinia hordei attack

GACR; PI P. Křenek

Mitogen-activated protein kinase-dependent phosphorylation and functional regulation of cytoskeletal End binding 1c protein

GACR; PI A. Kuchařová

Mitogen-activated protein kinase stress signalling in plants: from basic research in Arabidopsis towards biotechnological applications in crops

GACR; PI J. Šamaj; project with excelllent evaluation

Polarity control and cross-talk between signalling, endomembranes and the cytoskeleton in Arabidopsis root hairs

DFG Project, SA 1564/2-1; PI J. Šamaj; project with excelllent evaluation

The role and the integration of the cytoskeleton and MAPK signalling during osmotic and heat stress responses of higher plant cells

AvH Project; PI G. Komis

Cold and Salt Stress in plants regulated by endocytosis: from biochemistry and molecular cell biology to biotechnology

DAAD Project “Modern Applications of Biotechnology”; PI T. Takáč

The role of the cytoskeleton and vesicular trafficking in polarity establishement during in vitro embryogenesis and pollen development in Arabidopsis and maize

AvH Project; PI J. Šamaj

Key References

Hrbáčková M, Luptovčiak I, Hlaváčková K, Dvořák P, Tichá M, Šamajová O, Novák D, Bednarz H, Niehaus K, Ovečka M, and Šamaj J (2021) Overexpression of alfalfa SIMK promotes root hair growth, nodule clustering and shoot biomass production. Plant Biotechnology Journal 19, 767-784.

Ovečka M, Sojka J, Tichá M, Komis G, Basheer J, Marchetti C, Šamajová O, Kuběnová L, Šamaj J (2021) Imaging plant cells and organs with light-sheet and super-resolution microscopy. Plant Physiology, kiab349, doi: 10.1093/plphys/kiab349.

Tichá M, Illésová P, Hrbáčková M, Basheer J, Novák D, Hlaváčková K, Šamajová O, Niehaus K, Ovečka M, Šamaj J (2020) Tissue culture, genetic transformation, interaction with beneficial microbes, and modern bio-imaging techniques in alfalfa research, Critical Reviews in Biotechnology 40, 1265-1280.

Komis G, Šamajová O, Ovečka M, Šamaj J (2018) Cell and developmental biology of plant mitogen-activated protein kinases. Annual Review of Plant Biology 69, 237-265.

Ovečka M, von Wangenheim D, Tomančák P, Šamajová O, Komis G, and Šamaj J (2018) Multiscale imaging of plant development by light-sheet fluorescence microscopy. Nature Plants 4, 639-650.

Komis G, Novák D, Ovečka M, Šamajová O, Šamaj J (2017) Advances in imaging plant cell dynamics. Plant Physiology, published online November 2017, doi: 10.1104/pp.17.00962

Takáč T, Šamajová O, Luptovčiak I, Pechan T, Šamaj J (2017) Feedback microtubule control and microtubule-actin cross-talk in Arabidopsis revealed by integrative proteomic and cell biology analysis of KATANIN 1 mutants. Molecular & Cellular Proteomics 16, 1591-1609

Vyplelová P, Ovečka M, Šamaj J (2017) Alfalfa root growth rate correlates with progression of microtubules during mitosis and cytokinesis as revealed by environmental light-sheet microscopy. Frontiers in Plant Science 8:1870

Komis G, Luptovčiak I, Ovečka M, Samakovli D, Šamajová O, Šamaj J (2017) Katanin effects on dynamics of cortical microtubules and mitotic arrays in Arabidopsis thaliana revealed by advanced live-cell imaging. Frontiers in Plant Science 8:866

Takáč T, Vadovič P, Pechan T, Luptovčiak I, Šamajová O, Šamaj J (2016) Comparative proteomic study of Arabidopsis mutants mpk4 and mpk6. Scientific Reports 6:28306.

Komis G, Mistrík M, Šamajová O, Ovečka M, Bartek J, Šamaj J (2015) Superresolution live imaging of plant cells using structured illumination microscopy. Nature Protocols 10(8), 1248-63

Ovečka M, Vaškebová L, Komis G, Luptovčiak I, Smertenko A, Šamaj J (2015) Preparation of plants for developmental and cellular imaging by light-sheet microscopy. Nature Protocols 10(8), 1234-47

Komis G, Šamajová O, Ovečka M, Šamaj J (2015) Super-resolution Microscopy in Plant Cell Imaging. Trends in Plant Science, 20(12), 834-843

Komis G, Šamaj J (2014) Plant MAP Kinases. Methods and Protocols. ISBN 978-1-4939-0921-6, Springer, Berlin, Heidelberg, pp. 266

Smékalová V, Luptovčiak I, Komis G, Šamajová O, Ovečka M, Doskočilová A, Takáč T, Vadovič P, Novák O, Pechan T, Ziemann A, Košútová P, Šamaj J (2014) Involvement of YODA and mitogen activated protein kinase 6 in Arabidopsis post-embryogenic root development through auxin up-regulation and cell division plane orientation. New Phytologist 203, 1175-1193

Ovečka M, Takáč T, Komis G, Vadovič P, Bekešová S, Doskočilová A, Smékalová V, Luptovčiak I, Šamajová O, Schweighofer A, Meskiene I, Jonak C, Křenek P,Lichtscheidl I, Skultety L, Hirt H, Šamaj J (2014) Salt- induced subcellular kinase relocation and seeding susceptibility caused by overexpression of Medicago SIMKK in Arabidopsis. Journal of Experimental Botany 65(9), 2335-2350

Komis G, Mistrík M, Šamajová O, Doskočilová A, Ovečka M, Illés P, Bártek J, Šamaj J (2014) Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy. Plant Physiology 165 (1), 129-148

Takáč T, Šamajová O, Vadovič P, Pechan T, Košútová P, Ovečka M, Husičková A, Komis G, Šamaj J (2014) Proteomic and biochemical analyses show functional network of proteins involved in antioxidant defense of Arabidopsis anp2anp3 double mutant. J Proteome Res, 13, 5347-5361

Berson T, Wangenheim D, Takáč T, Šamajová O, Rosero A, Ovečka M, Komis G, Stelzer E H, Šamaj J (2014) Trans-Golgi network localized small GTPase RabA1d is involved in cell plate formation and oscillatory root hair growth. BMC Plant Biology 14, 252

Beck M, Komis G, Müller J, Menzel D, Šamaj J (2010) Arabidopsis homologs of Nucleus- and Phragmoplast-Localized Kinase 2 and 3 and Mitogen-Activated Protein Kinase 4 are essential for microtubule organization. Plant Cell 22(3), 755-771

Takáč T, Pechan T, Šamajová OOvečka M, Richter H, Eck C, Niehaus K, Šamaj J (2012) Wortmannin treatment induces changes in Arabidopsis root proteome and post-Golgi compartments. J Proteome Res 11 (6), 3127-3142

Takáč T, Pechan T, Richter H, Müller J, Eck C, Böhm N, Obert B, Ren H, Niehaus K, Šamaj J (2011) Proteomics on brefeldin A-treated Arabidopsis roots reveals profilin 2 as a new protein involved in the cross-talk between vesicular trafficking and the actin cytoskeleton. Journal of Proteome Research 10(2), 488-501

Müller J, Beck M, Mettbach U, Komis G, Hause G, Menzel D, Šamaj J (2010) Arabidopsis MPK6 is involved in cell division plane control during early root development, and localizes to the pre-prophase band, phragmoplast, trans-Golgi network and plasma membrane. Plant Journal 61, 234-248

Takáč T, Pechan T, Šamaj J (2011) Differential proteomics of plant development . J Proteomics 74(5), 577-588

Šamaj J , Baluška F, Voigt B, Schlicht M, Volkmann D, Menzel D (2004) Endocytosis, actin cytoskeleton and signaling. Plant Physiology 135, 1150-1161

Šamaj J, Read ND, Volkmann D, Menzel D, Baluška F (2005) The endocytic network in plants. Trends Cell Biol, 15, 425-433

Šamaj J, Müller J, Beck M, Böhm N, Menzel D (2006) Vesicular trafficking, cytoskeleton and signalling in root hairs and pollen tubes. Trends in Plant Science 11, 594-600

Beck M, Komis G, Ziemann A, Menzel D, Šamaj J (2011) Mitogen-activated protein kinase 4 is involved in the regulation 1 of mitotic and cytokinetic microtubule transitions in Arabidopsis thaliana. New Phytologist, 189(4), 1069-1083

Šamaj J , Ovecka M, Hlavacka A, Lecourieux F, Meskiene I, Lichtscheidl I, Lenart P, Salaj J, Volkmann D, Bögre L, Baluška F, Hirt H (2002) Involvement of the mitogen-activated protein kinase SIMK in regulation of root hair tip-growth. EMBO J. 21, 3296-3306

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