Laboratories

Equipment

Laser scanning confocal microscope Fluoview 1000

system specification

System includes:

Inverted system microscope Olympus IX 81

• Observation in transmitted light, Nomarsky DIC - condenser with long working distance
• Fluorescence - excitation with 100W Hg discharge lamp, fluorescent filters (WU, WG, NB, LSM)
• Motorized focusing and lens exchange system (10, 20, 40, 60, 100x)

Olympus FV 1000 imaging system

• Laser unit with 4 lasers with wavelengths in the range UV–RED (405–633nm) and AOTF laser combiner
• Light sources:
  • Red (He–Ne 633nm / 10mW),
  • Green (He–Ne 543nm / 1,5mW),
  • Blue (Ar 457nm, 488nm, 515nm / 30mW),
  • near UV (diode pumped, 405nm / 20mW)
• 3 detection channels for confocal fluorescence image detection + transmitted light detector/Nomarski DIC

Software FV10-ASW 3.0 Viewer

Micrograph viewer in OIB format; contact for confocal unit code required to download software: doc. RNDr. Michaela Sedlářová, Ph.D.

Software LCS Imaris 7.3.0.

Software for 3D/4D imaging and quantitative image analysis.

Optical anti-vibration table with pneumatic vibration damping system Envist Olymext

operating instructions

System for microphotography Olympus DP70

Fluorescence microscope Olympus BX60 / Stereomicroscope Olympus SZ40

Funding rules

Use of the microscope by staff of the Faculty of Science departments participating in its purchase (KB, KBBG, KBC, KEF)

The operation of the confocal microscope will be financed in advance by the Department of Botany, Faculty of Science, University of Olomouc, which will then re-account the costs, reduced by funds received from fees from other institutions (see below), every 3 months to the departments that participated in the purchase of the microscope (i.e. KB, KBBG, KBC, KEF), in proportion to the use of the device by the individual departments during the given period.

In the case of expensive repairs (e.g. overloading of lasers, etc.), the participating departments will agree on the method of their payment in proportion to the use of the device during the period since the last repair (replacement) of the relevant part of the microscope.

Fees for the use of the microscope by staff of other departments within the Faculty of Science and Faculty of Medicine UPOL and other institutions

In case of free capacity, the microscope will also be available to employees of other UP departments and other institutions, provided that they pay fees for the operating hours used. Departement of Botany will invoice users for fees in the amount corresponding to the number of operating hours used, with each half hour started being counted.

The rates will be different for UPOL employees and for employees of other workplaces:

• Departments of UPOL (except KB, KBBG, KBC, KEF): 400 CZK/hour
• Institutions outside UPOL (in this case, energy costs and the technician's salary will also be included in the costs): 600 CZK/hour

The above rates may be subject to change after approval by representatives of the Faculty of Science departments participating in the purchase of confocal microscope (KB, KBBG, KBC, KEF).

Contact

doc. RNDr. Michaela Sedlářová, Ph.D.

Department of Botany, Faculty of Science, Palacký University in Olomouc

Šlechtitelů 27

CZ-77900

Olomouc

Equipment

Partec PAS flow cytometer

excitation source: green laser Cobolt Samba (532 nm, 100 mW)

applications: genome size estimation using propidium iodide staining, GC:AT content analysis

 

Partec ML flow cytometer

excitation source: Partec UV LED kit (365 nm, 10 mW)

applications: ploidy level analysis using DAPI staining, GC:AT content analysis, flow cytometry seed screen (FCSS)

 

Beckman Coulter CytoFLEX flow cytometer

excitation sources: 2 lasers (nearUV and blue)

applications: genome size estimation using propidium iodide staining, ploidy level analysis using DAPI staining, GC:AT content analysis, pollen ploidy and nuclei number analysis