doc. RNDr. JITKA FRÉBORTOVÁ, Ph.D.
Telephone:
585634871, 585634795
E-mail:
Workplace:
CRH Rekombinantní proteiny
Org. unit:
CATRIN
Address:
Šlechtitelů 27 - objekt H1 (CRH)
Room number:
1.10
Profession:
vědecký pracovník
Activity:
Preparation and characterization of recombinant proteins. Studies on metabolism of plant hormones cytokinins in plants and bacteria, focused predominantly on cytokinin dehydrogenases and other enzymes involved in cytokinin metabolism. Antimicrobial peptides (preparation by heterologous expression and antimicrobial activity testing).
Selected publications
Králová M., Frébortová J., Pěnčík A., Frébort I.
Overexpression of Trp-related genes in Claviceps purpurea leading to increased ergot alkaloid production.
New Biotechnology.
2021.
(ČLÁNEK)
Hanosová H., Galuszka P., Frébortová J., Frébort I.
Parasitic fungus Claviceps as a source for biotechnological production of ergot alkaloids.
Biotechnology Advances.
2013.
(ČLÁNEK)
FRÉBORT I., KOWALSKA M., HLUSKA T., FRÉBORTOVÁ J., GALUSZKA P.
Darwin Review. Evolution of cytokinin biosynthesis and degradation.
Journal of Experimental Botany.
2011.
(ČLÁNEK)
ČLÁNEK
Mirzaee M., Osmani Z., Frébortová J., Frébort I.
Recent advances in molecular farming using monocot plants.
BIOTECHNOLOGY ADVANCES.
2022.
Frébortová J., Frébort I.
Biochemical and Structural Aspects of Cytokinin Biosynthesis and Degradation in Bacteria.
Microorganisms.
2021.
Torres D., Benavidez I., Iliana F., Mongiardini E., Rosas S., Spaepen S., Vanderleyden J., Pěnčík A., Novák O., Strnad M., Frébortová J., Cassan F.
New insights into auxin metabolism in Bradyrhizobium japonicum.
RESEARCH IN MICROBIOLOGY.
2018.
Saichana N., Tanizawa K., Ueno H., Pechoušek J., Novák P., Frébortová J.
Characterization of auxiliary iron-sulfur clusters in a radical S-adenosylmethionine enzyme PqqE from Methylobacterium extorquens AM1.
FEBS Open Bio.
2017.
Frébortová J., Plíhal O., Florová V., Kokáš F., Kubiasová K., Greplová M., Šimura J., Novák O., Frébort I.
Light influences cytokinin biosynthesis and sensing in Nostoc (cyanobacteria).
Journal of Phycology.
2017.
Hluska T., Dobrev PI., Tarkowská D., Frébortová J., Zalabák D., Kopečný D., Plíhal O., Kokáš F., Briozzo P., Zatloukal M., Motyka V., Galuszka P.
Cytokinin metabolism in maize: Novel evidence of cytokinin abundance, interconversions and formation of a new trans-zeatin metabolic product with a weak anticytokinin activity.
Plant Science.
2016.
Saichana N., Tanizawa K., Pechoušek J., Novák P., Yakushi T., Toyama H., Frébortová J.
PqqE from Methylobacterium extorquens AM1: a radical S-adenosyl-l-methionine enzyme with an unusual tolerance to oxygen.
Journal of Biochemistry.
2016.
Saichana N., Matsuschita K., Adachi O., Frébort I., Frébortová J.
Acetic acid bacteria: A group of bacteria with versatile biotechnological applications.
Biotechnology Advances.
2015.
Frébortová J., Greplová M., Seidl M., Heyl A., Frébort I.
Biochemical Characterization of Putative Adenylate Dimethylallyltransferase and Cytokinin Dehydrogenase from Nostoc sp PCC 7120.
PLoS One.
2015.
Zalabák D., Galuszka P., Mrízová K., Podlešáková K., Gu .., Frébortová J.
Biochemical characterization of the maize cytokinin dehydrogenase family and cytokinin profiling in developing maize plantlets in relation to the expression of cytokinin dehydrogenase genes.
Plant Physiology and Biochemistry.
2014.
Tarkowski P., Floková K., Václavíková K., Jaworek P., Raus M., Nordstroem A., Novák O., Doležal K., Šebela M., Frébortová J.
An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins.
Molecules.
2010.
Frébortová J., Novák O., Frébort I., Jorda R.
Degradation of cytokinins by maize cytokinin dehydrogenase is mediated by free radicals generated by enzymatic oxidation of natural benzoxazinones.
Plant Journal.
2010.
Frébortová J.
Function of plant defense secondary metabolite in cytokinin degradation.
Plant Signaling & Behavior (print).
2010.
Kowalska M., Galuszka P., Frébortová J., Šebela M., Bereš T., Hluska T., Šmehilová M., Bilyeu KD., Frébort I.
Vacuolar and cytosolic cytokinin dehydrogenases of Arabidopsis thaliana: heterologous expression, purification and properties.
Phytochemistry.
2010.
Galuszka P., Popelková H., Werner T., Frébortová J., Pospíšilová H., Mik V., Köllmer I., Schmülling T., Frébort I.
Biochemical characterization and histochemical localization of cytokinin oxidases/dehydrogenases from Arabidopsis thaliana expressed in Nicotiana tabaccum L.
Journal of Plant Growth Regulation.
2007.
Frébortová J., Galuszka P., Werner T., Schmülling T., Frébort I.
Functional expression and purification of cytokinin dehydrogenase from Arabidopsis thaliana (AtCKX2) in Saccharomyces cerevisiae.
Biologia Plantarum.
2007.
Popelková H., Fraaije M., Novák O., Frébortová J., Bilyeu KD., Frébort I.
Kinetic and chemical analyses of the cytokinin oxidase/dehydrogenase catalysed reaction: correlations with the crystal structure.
Biochemical Journal.
2006.
Frébortová J., Frébort I., Galuszka P., Werner T., Schmülling T.
Expression, purification and characterization of recombinant Arabidopsis thaliana cytokinin dehydrogenase AtCKX2.
Biologia Plantarum.
2005.
Frébort I., Popelková H., Fraaije W., Novák O., Frébortová J., Bilyeu KD.
Kinetic analysis and structural correlations of the reaction of cytokinin dehydrogenase (EC. 1.5.99.12).
Biologia Plantarum.
2005.
Galuszka P., Frébortová J., Luhová L., Bilyeu KD., English JT., Frébort I.
Tissue localization of cytokinin dehydrogenase in maize: Possible involvement of quinone species generated from plant phenolics by other enzymatic systems in the catalytic reaction.
Plant and Cell Physiology.
2005.
Frébort I., Galuszka P., Frébortová J., Popelková H., Drábek J.
Biochemistry and physiological functions of cytokinin dehydrogenase in plants.
In Frébort I., Walterová D. (Eds.)
2004.
Frébortová J., Fraaije MW., Galuszka P., Šebela M., Peč P., Hrbáč J., Novák O., Bilyeu KD., English JT., Frébort I.
Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors.
Biochemical Journal.
2004.
Galuszka P., Frébortová J., Werner T., Yamada M., Strnad M., Schmülling T., Frébort I.
Cytokinin oxidase/dehydrogenase genes in barley and wheat.
European Journal of Biochemistry.
2004.
Galuszka P., Frébortová J., Werner T., Yamada M., Strnad M., Schmuelling T., Frébort I.
Cytokinin oxidase/dehydrogenase genes in barley and wheat - cloning and heterologous expression.
European Journal of Biochemistry.
2004.
Frébortová J., Popelková H., Galuszka P., Werner T., Drábek J., Schmuelling T., Frébort I.
Expression of Arabidopsis thaliana cytokinin dehydrogenase in yeast Saccharomyces cerevisiae and tobacco plants.
Acta Physiologiae Plantarum.
2004.
Frébort I., Galuszka P., Frébortová J., Popelková H., Drábek J., Bilyeu KD.
Is the degradation of cytokinins in plants assisted by phenolics?
In Frébort I., Walterová D. (Eds.)
2004.
Frébort I., Galuszka P., Frébortová J., Popelková H., Drábek J., Bilyeu KD.
Possible involvement of plant phenolics in catalytic reaction of cytokinin dehydrogenase.
Acta Physiologiae Plantarum.
2004.
Sauer P., Frébortová J., Šebela M., Galuszka P., Jacobsen S., Peč P., Frébort I.
Xanthine dehydrogenase from pea seedlings: a member of plant molybdenum oxidoreductase family.
Plant Physiology and Biochemistry.
2002.
Bancířová M., Lasovský J., Kolářová H., Frébortová J., Medková J.
Chemiexcitation of phtalocyanines for the photodynamic therapy by phtalhydrazide on the gram-positive and gram-negative bacteria.
Bioluminescence and Chemiluminescence: Perspectives for the 21st Century, Proceedings of 10th ISBC John Wiley&Sons, Ltd., Chichester.
1999.
Bancířová M., Lasovský J., Kolářová H., Frébortová J., Medková J.
Chemiexcitation of phtalocyanines for the photodynamic therapy by phthalhydrazide on the Gram-positive and Gram-negative bacteria.
Journal of Bioluminescence and Chemiluminescence.
1998.
Frébortová J., Matsushita K.., Arata H.., Adachi O..
Intramolecular electron transport in quinoprotein alcohol dehydrogenase of Acetobacter methanolicus: a redox-titration study.
Biochimica et Biophysica Acta.
1998.
PŘÍSPĚVEK VE SBORNÍKU
Bancířová M., Lasovský J., Kolářová H., Frébortová J., Medková J.
Chemiexcitation of phthalocyanines for the photodynamic therapy by phthalhydrazide on the gram-positive and gram negative bacteria, Bioluminescence and Chemiluminescence. Perspectives for the 21 st Century.
Bioluminescence and Chemiluminescence: Perspectives for the 21st Century. John Wiley & Sons. Ltd. 1999.
1999.
KAPITOLA V KNIZE
Galuszka P., Spíchal L., Kopečný D., Tarkowski P., Frébortová J., Šebela M., Frébort I.
Metabolism of plant hormones cytokinins and their function in signaling, cell differentiation and plant development.
Studies in Natural Products Chemistry.
2008.
Frébortová J., Galuszka P., Frébort I.
Cytokinins and Plant Phenolics: A Cooperative Metabolism.
Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues.
2006.
Popelková H., Galuszka P., Frébortová J., Bilyeu KD., Frébort I.
Cytokinin dehydrogenase: characterization and structure homology modeling of the flavoprotein catabolizing plant hormones cytokinins.
Recent Research Developments in Proteins.
2004.
PATENT, UŽITNÝ VZOR, PRŮMYSLOVÝ VZOR
Werner T., Strnad M., Doležal K., Zatloukal M., Gemrotová M., Galuszka P., Schmülling T., Frébortová J., Spíchal L.
Substituted 6-anilinopurine derivatives as inhibitors of cytokinin oxidase/dehydrogenase and preparations containing these derivatives.
2014.
Spíchal L., Gemrotová M., Zatloukal M., Frébortová J., Galuszka P., Werner T., Schmulling T., Doležal K., Strnad M.
Substituted 6-anilinopurine derivatives as inhibitors of cytokinin oxidase/dehydrogenase and preparations containing these derivatives.
2013.
Spíchal L., Gemrotová M., Zatloukal M., Frébortová J., Galuszka P., Werner T., Schmülling T., Doležal K., Strnad M.
Substituted 6-anilinopurine derivatives as inhibitors of cytokinin oxidase/dehydrogenase and preparations containing these derivatives.
2012.
SPÍCHAL L., GEMROTOVÁ M., ZATLOUKAL M., FRÉBORTOVÁ J., GALUSZKA P., Werner T., Schmulling T., DOLEŽAL K., STRNAD M.
Substituted 6-anilinopurine derivatives as inhibitors of cytokinin oxidase/dehydrogenase and preparations containing these derivatives.
2011.
STRNAD M., SPÍCHAL L., GEMROTOVÁ M., ZATLOUKAL M., FRÉBORTOVÁ J., GALUSZKA P., Werner T., Schmulling T., DOLEŽAL K.
Substituted 6-anilinopurine derivatives as inhibitors of cytokinin oxidase/dehydrogenase and preparations containing these derivatives.
2011.
Spíchal L., Gemrotová M., Zatloukal M., Frébortová J., Galuszka P., Werner T., Schmülling T., Doležal K., Strnad M.
Substituované 6-anilinopurinové deriváty jako inhibitory cytokinin oxidasy a přípravky obsahující tyto sloučeniny.
2010.
POLOPROVOZ, TECHNOLOGIE
Havlíček L., Doležal K., Novák O., Frébortová J., Strnad M.
Production technology for preparation of [15N4] hypoxanthine.
2010.
ABSTRAKT
Frébortová J., Novák O., Jorda R.
Role of benzoxazinones in the activation of maize cytokinin dehydrogenase.
Physiologia Plantarum.
2008.
Popelková H., Fraaije MW., Novák O., Frébortová J., Bilyeu KD.
Kinetic analysis of the cytokinin oxidase/dehydrogenase reaction.
Book of Abstracts, 15th International Symposium on Flavins and Flavoproteins.
2005.
Bancířová M., LASOVSKÝ J., Kolářová H., Medková J., Frébortová J.
Chemiexcitation of phtalocyanines for the photodynamic therapy by phthalhydrazide on the gram-positive and gram-negative bacteria.
Sborník abstraktů 10th International Symphosium on Bioluminescence and Chemiluminescence, Bologna, Italy.
1998.
ELEKTRONICKÁ PREZENTACE
Bancířová M., Kolářová H., Lenobel R., Frébortová J., Medková J., Lasovský J.
THE CHEMIEXCITATION OF THE PHOTOSENSITIZERS.
Internet Journal of Photochemistry and Photobiology.
2000.
Bancířová M., Kolářová H., Lenobel R., Frébortová J., Medková J., Lasovský J.
The chemiexcitations of the photosensitizers.
Internet Journal of Photochemistry and Photobiology.
2000.
ZPRÁVA
Strnad M., Spíchal L., Doležal K., Ulbrichová M., Frébortová J., Galuszka P., Zatloukal M.
Smluvní výzkum.
2020.
Current courses
| Course title | Department/Abbrev. | Semester | Type | Classes |
|---|---|---|---|---|
| Microbiology | LRR/MB | LS | Př | 3 |
| Principles and Applications of Molecular Biotechnology | LRR/MBT | ZS | Př | 2 |
| Microbiology | KCB/MB | LS | Př | 3 |
Doctoral study on UP
| Name | Faculty | Study programme | Year of completion |
|---|---|---|---|
| ZALABÁK | PRF | Biochemie | 2013 |
| VRABKA | PRF | Biochemie | 2019 |
List contains doctoral study graduates